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1:5 Dilution (1mL Total Volume)
1. Press repeatedly until the
display reads:
2. Press to enter selection.
3. Attach a pipette tip to the tip cone.
The pipettor is now ready to begin the
1:5 dilution (1mL total volume). The
display indicates that the pipettor is
ready to aspirate 0.80mL.
4. Place the tip in the diluent and press the
Start Button. The unit will aspirate
0.80mL.
The following display indicates that the
unit is ready to aspirate 0.20mL.
5. Place the tip in the sample solution
and press the Start Button to aspirate
0.20mL.
The next display indicates that the unit
is ready to dispense the total volume
(1mL) with blowout.
6. Position the pipette tip to dispense and
press the Start Button. The unit will
dispense the full amount from the tip.
The display now indicates that the unit
is ready to repeat the dilution sequence,
beginning with the 0.80mL aspiration.
12
Pipettor Programming
Pipettor Programming
11
1:20 Dilution (5mL Total Volume)
1. Press repeatedly until the
display reads:
2. Press to enter selection.
3. Attach a pipette tip to the tip cone.
The pipettor is now ready to begin the
1:20 dilution (5mL total volume). The
display indicates that the pipettor is
ready to aspirate 4.75mL.
4. Place the tip in the diluent and press the
Start Button. The unit will aspirate
4.75mL.
The following display indicates that the
unit is ready to aspirate 0.25mL.
5. Place the tip in the sample solution
and press the Start Button to aspirate
0.25mL.
The next display indicates that the unit
is ready to dispense the total volume
(5mL) with blowout.
6. Position the pipette tip to dispense and
press the Start Button. The unit will
dispense the full amount from the tip.
The display now indicates that the unit
is ready to repeat the dilution sequence,
beginning with the 4.75mL aspiration.
Page 6 of 15 - June 2001